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    Type 3 Secretion Systems: Methods and Protocols - Molecular Biology - PAPERBACK

    • Item No : 127285589552
    • Condition : Very Good
    • Brand : No brand Info
    • Seller : lorrzamb-0
    • Current Bid : US $12.95

    • * Item Description

    • Type 3 Secretion Systems: Methods and Protocols - Molecular Biology - PAPERBACK

      **Corners have some Creases - Pages inside are like new no writing or marks - Please see all Images**

      This volume discusses various basic and advanced methods and protocols that have been proven to be successful among certain bacterial species, or a family of species, in type III secretion systems (T3S system). The chapters in this book cover topics such as: site-directed mutagenesis and its application in studying the interactions of T3S components; use of transcriptional control to increase expression and secretion of heterologous proteins in T3S systems; fractionation techniques to examine effector translocation; detecting immune responses to T3S systems; mouse immunization with purified needle proteins from T3S systems and the characterization of the immune responses to these proteins; and detection of protein interactions in T3S systems using yeast 2-hybrid analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

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