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Two-Hybrid Systems : Methods and Protocols, Paperback by Oñate-sánchez, Luis ...
- Item No : 197214411742
- Condition : Like New
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- Seller : greatbookprices2
- Current Bid : US $170.56
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* Item Description
- Two-Hybrid Systems : Methods and Protocols, Paperback by Oñate-sánchez, Luis (EDT), ISBN 1493993100, ISBN-13 9781493993109, Like New Used, Free shipping in the US
This detailed book explores the numerous applications of the yeast two-hybrid (Y2H) system, one of the most commonly used methods to detect protein-protein interactions. Beginning with an overview, the volume then continues by elucidating different methodologies to detect protein-protein interactions in yeast nucleus, membrane, cytoplasm, and bacteria, computational analyses of protein interaction networks, technical variations in yeast one-hybrid (Y1H) systems together with classical and more elaborated yeast methods to detect protein-DNA interactions, as well as protocols to analyze ternary protein interactions and RNA-protein and ligand-protein interactions. Th concludes with a section on methods in plant and mammalian cells that can be used to identify novel protein-protein and protein-DNA interactions and test (qualitatively and/or quantitatively) those observed in yeast. Written for the highly successful <i>Methods in Molecular Biology</i> series, chapters include the kind of detail and key implementation advice that leads to excellent results in the lab. <div><br></div><div>Authoritative and practical, <i>Two-Hybrid Systems: Methods and Protocols</i> serves as an ideal guide to the uses of this exceedingly valuable technique.</div>
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