Protein Terminal Profiling: Methods and Protocols by Oliver Schilling (English)

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This volume presents detailed protocols for novel strategies and approaches to improve functional understanding of protein N- and C-terminal biology. Protein Terminal Profiling: Methods and Protocols addresses topics such as protease specificity profiling, N-terminal acetylation, assays to probe protease activity in cellular systems, protein N- and C-termini on a proteome-wide scale, and biochemical approaches to explain and examine extracellular protease activities. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. < Cutting-edge and thorough, Protein Terminal Profiling: Methods and Protocols is a valuable resource for researchers that focus on biochemistry and cell biology, and those who share a broad interest in protein functionality and protein modifications.

Table of Contents

[14C]-Acetyl-CoenzymeA-Based In Vitro N-Terminal Acetylation Assay.- DTNB-Based Quantification of In Vitro Enzymatic N-Terminal Acetyltransferase Activity.- SILProNAQ: A Convenient Approach for Proteome-Wide Analysis of Protein N-Termini and N-Terminal Acetylation Quantitation.- Profiling of Protein N-Termini and their Modifications in Complex Samples.- Protease Substrate Profiling by N-Terminal COFRADIC.- Doublet N-Terminal Oriented Proteomics for N-Terminomics and Proteolytic Processing Identification.- Multidimensional Analysis of Protease Substrates and their Cellular Origins in Mixed Secretomes from Multiple Cell Types.- System-Wide Profiling of Protein Amino Termini from Formalin-Fixed, Paraffin-Embedded Tissue Specimens for the Identification of Novel Substrates.- Identification of Carboxypeptidase Substrates by C-Terminal COFRADIC.- ProC-TEL: Profiling of Protein C-Termini by Enzymatic Labeling.- Determining Protease Substrates within a Complex Protein Background using the PROtein TOpography and Migration Analysis Platform (PROTOMAP).- Multiplexed Protease Specificity Profiling Using Isobaric Labeling.- FPPS - Fast Profiling of Protease Specificity.- Profiling of Protease Cleavage Sites by Proteome-Derived Peptide Libraries and Quantitative Proteomics.- Prediction of Proteases Involved in Peptide Generation.- Live-Cell Imaging of Protease Activity: Assays to Screen Therapeutic Approaches.- Protein Translocation Assays to Probe Protease Function and Screen for Inhibitors.- Simultaneous Detection of Metalloprotease Activities in Complex Biological Samples Using the PrAMA (Proteolytic Activity Matrix Assay) Method.- Synthesis and Application of Activity-Based Probes for Proteases.

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Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

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