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  • High-Resolution Imaging of Cellular Proteins: Methods and Protocols by Steven D.

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      High-Resolution Imaging of Cellular Proteins

      by Steven D. Schwartzbach, Omar Skalli, Thomas Schikorski

      This volume presents authoritative and cutting-edge methods and protocols focusing on three tool boxes covering the increasingly diverse methodologies used to image selected proteins and to investigate their function by light and electron microscopy.

      FORMAT
      Paperback
      LANGUAGE
      English
      CONDITION
      Brand New


      Publisher Description

      This volume presents authoritative and cutting-edge methods and protocols focusing on three tool boxes covering the increasingly diverse methodologies used to image selected proteins and to investigate their function by light and electron microscopy. The first tool box includes the development of a wide range of molecular and immunological probes to target specific proteins. The second details the use of these probes for high resolution fluorescence microscopy and the third focuses on applications for transmission and scanning electron microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls thus ensuring successful results in the further study of this vital field.

      Back Cover

      This volume presents authoritative and cutting-edge methods and protocols focusing on three tool boxes covering the increasingly diverse methodologies used to image selected proteins and to investigate their function by light and electron microscopy. The first tool box includes the development of a wide range of molecular and immunological probes to target specific proteins. The second details the use of these probes for high resolution fluorescence microscopy and the third focuses on applications for transmission and scanning electron microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls thus ensuring successful results in the further study of this vital field.

      Table of Contents

      Expression of Epitope-Tagged Proteins in Mammalian Cells in Culture.- Antibody Production with Synthetic Peptides.- Production and Purification of Polyclonal Antibodies.- Preparation of Colloidal Gold Particles and Conjugation to Protein A/G/L, IgG, F(ab')2 and Streptavidin.- Helper Dependent Adenoviral Vectors and their use for Neuroscience Applications.- Localizing Proteins in Fixed Giardia Lamblia and Live Cultured Mammalian Cells by Confocal Fluorescence Microscopy.- Using Fluorescent Protein Fusions to Study Protein Subcellular Localization and Dynamics in Plant Cells.- Using FRAP Or FRAPA to Visualize the Movement of Fluorescently-Labeled Proteins or Cellular Organelles in Live Cultured Neurons Transformed with Adeno-Associated Viruses.- Bimolecular Fluorescence Complementation (BiFc) Analysis of Protein-Protein Interactions and Assessment of Subcellular Localization in Live Cells.- Viral Injection and Cranial Window Implantation for In Vivo Two-Photon Imaging.- Imaging SynapticVesicle Exocytosis-Endocytosis with pH Sensitive Fluorescent Proteins.- Immunogold Protein Localization on Grid-Glued Freeze-Fracture Replicas.- Pre-Embedding Double-Label Immunoelectron Microscopy of Chemically Fixed Tissue Culture Cells.- Immunoelectron Microscopy of Cryofixed and Freeze- Substituted Plant Tissues.- Immunoelectron Microscopy of Cryofixed Freeze Substituted Yeast Saccharomyces cerevisiae.- Pre-embedding Method of Electron Microscopy for Glycan Localization in Mammalian Tissues and Cells Using Lectin Probes.- Pre-Embedding Nanogold Silver and Gold Intensification.- Post-Embedding Mammalian Tissue for Immunoelectron Microscopy: A Standardized Procedure Based On Heat-Induced Antigen Retrieval.- Pre- and Post-Embedding Immunogold Labeling of Tissue Sections.- Immuno-Gold Labelling for Scanning Electron Microscopy.- Monitoring Synaptic Vesicle Protein Sorting with Enhanced Horseradish Peroxidase in the Electron Microscope.- Functional Nanoscale Imaging Of Synaptic VesicleCycling With Superfast Fixation.

      Review

      "This is a collection of timely techniques and methods that have been key for the development of imaging at the structural and ultrastructural level. … The intended audience is cell biologists and all scientists working with imaging techniques to localize protein expression at a cellular and subcellular level. … Each chapter ends with a list of references. Graphics and high-quality figures are displayed where needed." (Michele Fornaro, Doody's Book Reviews, July, 2017)

      Review Quote

      "This is a collection of timely techniques and methods that have been key for the development of imaging at the structural and ultrastructural level. ... The intended audience is cell biologists and all scientists working with imaging techniques to localize protein expression at a cellular and subcellular level. ... Each chapter ends with a list of references. Graphics and high-quality figures are displayed where needed." (Michele Fornaro, Doody's Book Reviews, July, 2017)

      Feature

      Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

      Details

      ISBN1493981730
      Pages 366
      Publisher Humana Press Inc.
      Series Methods in Molecular Biology
      Year 2018
      ISBN-10 1493981730
      ISBN-13 9781493981731
      Format Paperback
      Publication Date 2018-06-09
      Imprint Humana Press Inc.
      Subtitle Methods and Protocols
      Place of Publication Totowa, NJ
      Country of Publication United States
      Edited by Thomas Schikorski
      DEWEY 616.0757
      Short Title High-Resolution Imaging of Cellular Proteins
      Language English
      Series Number 1474
      UK Release Date 2018-06-09
      AU Release Date 2018-06-09
      NZ Release Date 2018-06-09
      US Release Date 2018-06-09
      Author Thomas Schikorski
      Birth 1957
      Affiliation University of Minnesota School of Law
      Position EDFRTR
      Qualifications Sir
      Edition Description Softcover reprint of the original 1st ed. 2016
      Alternative 9781493963508
      Audience Professional & Vocational
      Illustrations 21 Illustrations, color; 24 Illustrations, black and white; XIII, 366 p. 45 illus., 21 illus. in color.

      TheNile_Item_ID:130978723;
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