Cell Microencapsulation: Methods and Protocols: 2016 (Methods in Molecular

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This volume provides a unique forum to review cell microencapsulation in a broad sense by exploring various cell types that have been encapsulated for different purposes, different approaches and devices used for microencapsulation, the biomaterials used in cell microencapsulation, the challenges to the technology, and the current status of its application in different clinical situations. This book is divided in five sections: Section I is an introductory part that discusses historical developments of the technology and its current challenges, as well as the various applications of cell microencapsulation; Section II discusses the main approaches and devices currently used in cell microencapsulation; Section III presents an overview of the various polymeric materials currently in use for cell microencapsulation and the enabling technologies to either monitor or enhance encapsulated cell function; Section IV gives specific examples of the methods used to encapsulate various cell types; and Section V provides an overview of the different clinical situations in which cell microencapsulation has been applied. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Thorough and practical, Cell Microencapsulation: Methods and Protocols is a valuable reference for researchers, engineers, clinicians, and other healthcare professionals, as well as food technologists who will find detailed descriptions of methods for the microencapsulation of specific cell types and their current of potential clinical and industrial applications. This volume also includes detailed information about the design and manufacture of different devices including large-scale production devices for use in cell microencapsulation.

Part I Introduction

1. Historical Perspectives and Current Challenges in Cell Microencapsulation Paul de Vos 2. Applications of Cell Microencapsulation Emmanuel C. Opara Part II Approaches to Cell Microencapsulation 3. Cell Microencapsulation: Dripping Methods A. Bidoret, E. Martins, B. Poncelet De Smet, and D. Poncelet 4. Field Effect Microparticle Generation for Cell Microencapsulation Brend Ray-Sea Hsu and Shin-Huei Fu 5. Microfluidic Approach to Cell Microencapsulation Varna Sharma, Michael Hunckler, Melur K. Ramasubramanian, Emmanuel C. Opara, and Kalyan C. Katuri Part III Biomaterials and Enabling Technologies in Cell Microencapsulation 6. Polymeric Materials for Cell Microencapsulation A. Aijaz, D. Perera, and R. Olabisi

16. Use of Flow Focusing Technique for Microencapsulation of Myoblasts J. Ciriza, L. Saenz del Burgo, RM Hernandez, G. Orive, and JL Pedraz

17. Alginate Microbeads for Cell and Protein Delivery Sami I. Somo, Omaditya Khanna, and Eric M. Brey

18. Compartmentalization of Two Cell Types in Multi-Layered Alginate Microcapsules Sivanandane Sittadjody, Justin M. Saul, and Emmanuel C. Opara 19. Primary Choroid Plexus Tissue for Use in Cellular Therapy M.A. Sandrof and C.G. Thanos 20. Microencapsulation of Stem Cells for Therapy Shirae K. Leslie, Ramsey C. Kinney, Zvi Schwartz, and Barbara D. Boyan