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  • Argonaute Proteins: Methods and Protocols by Katsutomo Okamura (English) Hardcov

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      Argonaute Proteins

      by Katsutomo Okamura, Kotaro Nakanishi

      This volume covers methods that analyze various Argonaute proteins from a variety of organisms to help researchers better understand their properties ranging from a molecular level to an organismal level.

      FORMAT
      Hardcover
      LANGUAGE
      English
      CONDITION
      Brand New


      Publisher Description

      This volume covers methods that analyze various Argonaute proteins from a variety of organisms to help researchers better understand their properties ranging from a molecular level to an organismal level. The chapters in this book explore the following topics: identification and expression analysis of guide nucleic acids and their targets; analysis of biochemical properties of Argonautes; biological functions of Argonautes; and obtaining materials and setting up analysis platforms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Argonaute Proteins: Methods and Protocols is a valuable resource for researchers and scientists looking to expand their knowledge ofArgonaute proteins and their functions.

      Back Cover

      This volume covers methods that analyze various Argonaute proteins from a variety of organisms to help researchers better understand their properties ranging from a molecular level to an organismal level. The chapters in this book explore the following topics: identification and expression analysis of guide nucleic acids and their targets; analysis of biochemical properties of Argonautes; biological functions of Argonautes; and obtaining materials and setting up analysis platforms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Argonaute Proteins: Methods and Protocols is a valuable resource for researchers and scientists looking to expand their knowledge of Argonaute proteins and their functions.

      Table of Contents

      Cloning and Identification of Recombinant-Argonaute Bound Small RNAs using Next-Generation Sequencing.- Quantification of miRNAs Co-Immunoprecipitated with Argonaute Proteins using SYBR Green-Based qRT-PCR.- Gateway to Understanding Argonaute Loading of Single-Stranded RNAs: Preparation of Deep Sequencing Libraries with In Vitro Loading Samples.- Dumbbell-PCR for Discriminative Quantification of a Small RNA Variant.- MicroRNA Detection by Whole-Mount In Situ Hybridization in C. elegans.- cCLIP-Seq: Retrieval of Chimaeric Reads from HITS-CLIP (CLIP-Seq) Libraries.- Kinetic Analysis of Small Silencing RNA Production by Human and Drosophila Dicer Enzymes In Vitro.- Nucleic Acid-Binding Assay of Argonaute Protein using Fluorescence Polarization.- Reconstitution of RNA Interference Machinery.- Single-Molecule Analysis for RISC Assembly and Target Cleavage.- Profiling Open Chromatin Structure in the Ovarian Somatic Cells using ATAC-Seq.- Assessing miR-451 Activity and Its Role in Erythropoiesis.- Functional Analysis of MicroRNAs in Neurogenesis during Mouse Cortical Development.- Cellular Approaches in Investigating Argonaute2-Dependent RNA Silencing.- Genomic Tagging of AG01 using CRISPR/Cas9-Mediated Homologous Recombination.- Accurate Profiling and Quantification of tRNA Fragments from RNA-Seq Data: A vade mecum for MINTmap.

      Feature

      Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

      Details

      ISBN149397338X
      Publisher Humana Press Inc.
      Year 2017
      ISBN-10 149397338X
      ISBN-13 9781493973385
      Format Hardcover
      Edition 1st
      Imprint Humana Press Inc.
      Subtitle Methods and Protocols
      Place of Publication Totowa, NJ
      Country of Publication United States
      Edited by Kotaro Nakanishi
      DEWEY 572.6
      Illustrations 44 Illustrations, color; 11 Illustrations, black and white; XIII, 260 p. 55 illus., 44 illus. in color.
      Pages 260
      Language English
      Media Book
      Publication Date 2017-10-18
      Short Title Argonaute Proteins
      DOI 10.1007/978-1-4939-7339-2
      Series Number 1680
      AU Release Date 2017-10-18
      NZ Release Date 2017-10-18
      US Release Date 2017-10-18
      UK Release Date 2017-10-18
      Author Kotaro Nakanishi
      Edition Description 1st ed. 2018
      Series Methods in Molecular Biology
      Alternative 9781493984572
      Audience Professional & Vocational

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