Abscisic Acid: Methods and Protocols (Methods in Molecular Biology) [Hardback]

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This volume presents an all-inclusive overview of molecular biological methods to study ABA signaling transport, and metabolism in several plant species. The chapters in this book cover topics such as biochemical and molecular biological characterization of ABA receptors; assay systems for ABA transporters; quantification and visualization of ABA levels; and comprehensive omics approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Cutting-edge and thorough, Abscisic Acid: Methods and Protocols is a valuable resource for scientists and researchers who are interested in learning more about this developing field.

Acknowledgements... Preface... Table of Contents... Contributing Authors... 1 Inhibition of Type 2C Protein Phosphatases by ABA Receptors in Abscisic Acid-Mediated Plant Stress Responses Yeongmok Lee, Suhyeon Heo, and Sangho Lee 2 Inhibition of SnRK2 Kinases by Type 2C Protein Phosphatases Ewa Krzywinska, Katarzyna Patrycja Szymanska, and Grazyna Dobrowolska 3 Reconstitution of the Core ABA Signaling in Protoplasts: Transcriptional Activators Myung Ki Min, Rigyeong Kim, and Beom-Gi Kim 4 Affinity Purification of Ubiquitinated Proteins Using p62-Agarose to Assess Ubiquitination of Clade A PP2Cs Jose Julian, Alberto Coego, Abdulwahed F. Alrefaei, and Pedro L. Rodriguez 5 Microscopic Imaging of Endosomal Trafficking of ABA Receptors Borja Belda-Palazón and Pedro L. Rodriguez 6 ABA Transport Assay in Plant Single Cell System Joohyun Kang 7 Screening of ABA Transporters by A Yeast Two-Hybrid System-Based Screening using the Receptor Complex as a Sensor Shunsuke Watanabe, Yuri Kanno, and Mitsunori Seo 8 Use of Micrografting to Study the Role Played by Peptide Signals in ABA Biosynthesis Fuminori Takahashi 9 Gas Exchange Measurements in Systemic Signaling Studies Sanna Ehonen and Maija Sierla 10 Liquid Chromatography-Tandem Mass Spectrometry-Based Profiling of Plant Hormones Mohamed A. Salem and Ahmed Zayed 11 Live-Imaging of Abscisic Acid Dynamics using Genetically Encoded Fluorescence Resonance Energy Transfer (FRET)-Based ABA Biosensors Rainer Waadt 12 In Situ Observation of Abscisic Acid Distribution in Major Crop Species by Immunofluorescence Labeling Mo-Xian Chen, Chong-Chong Lu, Jianhua Zhang, and Ying-Gao Liu 13 Phosphoproteomic Approaches to Evaluate ABA Signaling Kota Yamashita and Taishi Umezawa 14 Affinity Purification followed by Liquid Chromatography-Tandem Mass Spectrometry to Identify Proteins Interacting with ABA Signaling Components Fumiyuki Soma, Fuminori Takahashi, Kazuo Shinozaki, and Kazuko Yamaguchi-Shinozaki 15 SWATH-MS Proteomic Approach to Discover Novel Protein Targets and Pathways in Response to Abscisic Acid Bei Gao, Mo-Xian Chen, and Fu-Yuan Zhu 16 Genome-Wide Association Analyses to Identify SNPs Related to Drought Tolerance Shengxue Liu and Feng Qin 17 Chemical Approaches for Improving Plant Water Use Aditya S. Vaidya and Sean R. Cutler Subject Index List...